Acta Botanica Croatica, Vol. 39 No. 1, 1980.
Izvorni znanstveni članak
Plantlet Regeneration from Shoot Tip Culture of Pelargonium zonale Hybrid
Sibila Jelaska
; Hrvatska
Biserka Jelenčić
; Hrvatska
Sažetak
Shoot tips (0.5 mm high) of Pelargonium zonale hybrid cvs. ’Career’ and ’Fire flash’ were cultured on modified MS medium: macroelements (Murashige and Skoog 1962), microelements (Nitsch and Nitsch 1970) containing (mg 1^1) thiamine.HCl 0.4, m-inositol 200, caseine hydrolysate 1000, IAA 0.5, kinetin 10.0; (g 1-1) sucrose 30, bacto agar 6; pH 5.8.
The ’Career’ did not grow on this medium, while the ’Fire flash’ did: 30% of shoots produced an organogenic callus, which was capable of being continuously subcultured. The shoots and plantlets obtained rooted well on a medium containing 0.1 mg 1-1 IAA only, and after a month they were transferred into pots. Callus cultures and in vitro formed plants, examined for Xanthomonas pelargonii and other bacteria, were found to be free of pathogens.
Ključne riječi
Hrčak ID:
158367
URI
Datum izdavanja:
31.12.1980.
Posjeta: 1.021 *