Acta Botanica Croatica, Vol. 40 No. 1, 1981.
Izvorni znanstveni članak
Electrophoretic Investigation of Some Grapevine Fanleaf Virus Isolates
Marija Vrdoljak
; Hrvatska
Ana Šarić
; Hrvatska
Sažetak
Grapevine fanleaf virus (GFV) isolates originating from the island of Vis were examined by agar-gel immunoelectrophoresis and by sodium dodecyl sulphate polyacrilamide (SDS-PAA) gel electrophoresis. Immunoelectrophoresis of the majority of isolates examined usually displayed two, sometimes three, electrophoretical components moving towards the cathode. Three centrifugal components from the density sucrose gradient had different velocities of migration. The top component was the fastest and the bottom was the slowest. Some strains including Berck s’ GFV isolate moved more slowly towards the cathode.
SDS-PAA gel electrophoresis of the grapevine isolates studied, in 7.5 and 10°/o slab and column gels, revealed one well defined band of grapevine fanleaf virus protein subunit which corresponded to the molecular weight of c. 53,700 daltons. In electrophoresis GFV protein migrated similarly to the cherry leaf roll virus protein. From the OO isolate, which had a wide herbaceous host range, unusual for GFV, the GFV band of about 52,000 and an additional prominent band of 41,000 daltons were obtained. In other isolates, besides the GFV protein subunit band, some less prominent bands were found.
Ključne riječi
Hrčak ID:
158425
URI
Datum izdavanja:
31.12.1981.
Posjeta: 814 *