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Effects of exogenous glutathione on suspension callus cells of spruce [Picea abies (L.) Karst.]

Maria Müller ; Institute of Plant Physiology, University of Graz, Austria
Bernd Zechmann ; Institute of Plant Physiology, University of Graz,
Michael Tausz ; Institute of Plant Physiology, University of Graz,
Karl Brendle ; Institute of Plant Physiology, University of Graz,
Günther Zellnig ; Institute of Plant Physiology, University of Graz,

Puni tekst: engleski pdf 2.476 Kb

str. 197-209

preuzimanja: 325



Callus cells of Picea abies (L.) Karst, were exposed to different concentrations (50, 100, 500, 1000 μM) of reduced glutathione (GSH) for 48 hours. These physiologically relevant concentrations of glutathione caused changes in the investigated tissue depending on the concentration applied. Feeding of glutathione increased the cellular concentrations of thiols, decreased the rate of cell division, induced mitotic abnormalities, generated increased amounts of micronuclei and affected the cell ultrastructure. The glutathione system in the callus culture cells was measured by a quantitative image analysis method, using histochemical staining by monochlorobimane. This measurement showed an increase of thiols at the cellular level after GSH treatment. Whereas no distinct structural modifications were found in cells treated with 50 and 100 μM, the treatment with 500 and 1000 μM GSH had multiple effects on the investigated tissue in comparison to control cells. Damage observed in the electron microscope involved separation of the plasma membrane from the cell wall, swollen plastids and mitochondria, and heavily granulated chromatin in the nuclei. The investigation of the chromosomal aberrations showed an increased amount of chromosomal defects in the GSH treated cells. The chromosomal aberration types observed most frequently were defects in the form of sticky chromosomes and vagrant chromosomes indicating severe damages in the genetic material.

Ključne riječi

Glutathione; callus; Picea abies; monochlorobimane; ultrastructure; chromosomal aberrations

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