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https://doi.org/10.5562/cca3135

Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Ethyl Ester (NACET) Generating Chromogenic Copper(I)L_n Complexes with Different Ligands

Lea Kukoc-Modun orcid.org/0000-0003-1798-6796 ; Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, HR-21000 Split, Croatia
Dimitrios Tsikas ; Center of Pharmacology and Toxicology, Hannover Medical School, Carl-Neuberg-Str. 1, DE-30625 Hannover, Germany
Tomislav Kraljević ; Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, HR-21000 Split, Croatia
Maja Biocic orcid.org/0000-0002-2293-3480 ; Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, HR-21000 Split, Croatia
Njegomir Radić ; Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, HR-21000 Split, Croatia

Sažetak

Three simple, sensitive and robust kinetic spectrophotometric methods for the determination of a novel lipophilic thiol compound, N-acetyl-L-cysteine ethyl ester (NACET), have been developed and validated. The methods are based on the reduction of Cu(II)-ligand complex to Cu(I)-ligand complex with the analyte. Studied ligands were neocuproine, bicinchoninic acid (BCA) and bathocuproine disulfonic acid (BCS). The development of chromogenic complexes was followed using kinetic setup with spectrophotometric detection at 458, 562 and 483 nm for the reactions of NACET with neocuproine, BCA and BCS, respectively. The calculated reaction orders with respect to NACET concentration were found to be 1.07, 1.01, 1.07, respectively, thus confirming a first order of reaction. The initial rate and fixed time methods were utilized for constructing calibration curves. Assays limits of detection were 1.4 × 10–7, 3.2 × 10−7 and 6.0 × 10−8 mol L–1, respectively. The analytical performance of the methods, in terms of accuracy and precision, was established.

This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi

N-acetyl-L-cysteine ethyl ester; kinetic spectrophotometry; neocuproine; bicinchoninic acid; bathocuproine disulfonic acid

Hrčak ID:

185729

URI

https://hrcak.srce.hr/185729

Datum izdavanja:

3.7.2017.

Posjeta: 2.267 *