Izvorni znanstveni članak

https://doi.org/10.2478/botcro-2018-0022

Genetic structure of populations of several endangered and endemic Dianthus species revealed by microsatellite markers

Anca Butiuc-Keul orcid.org/0000-0002-6523-6845 ; Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, 400084 Cluj-Napoca, Romania
Cornelia Crăciunaș ; Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, 400084 Cluj-Napoca, Romania
Irina Goia ; Department of Taxonomy and Ecology, Faculty of Biology and Geology, Babeş-Bolyai University, 400015 Cluj-Napoca, Romania
Anca Farkas ; Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, 400084 Cluj-Napoca, Romania
Liliana Jarda ; Alexandru Borza Botanical Garden, Babeş-Bolyai University, 400015 Cluj-Napoca, Romania
Victoria Cristea ; Alexandru Borza Botanical Garden, Babeş-Bolyai University, 400015 Cluj-Napoca, Romania

Sažetak

In order to develop a proper conservation programme for several endangered, rare or endemic species of Dianhtus from Romania, molecular characterization by simple sequence repeat (SSR) markers has been accomplished. Amplification of SSR loci in individuals belonging to different populations of D. callizonus, D. glacialis ssp. gelidus, D. henteri, D. nardiformis and D. tenuifolius revealed 23 polymorphic alleles. D. callizonus and D. tenuifolius showed particular sets of SSR alleles. D. glacialis ssp. gelidus, D. henteri and D. nardiformis proved to share almost the same alleles in most of the loci. The highest genetic diversity was observed in D. glacialis ssp. gelidus and D. tenuifolius in locus MS-DINMADSBOX. Allelic patterns across Dianthus species indicate that the mean number of different alleles was highest in D. glacialis ssp. gelidus, while the number of effective alleles was highest in D. tenuifolius. There are no particular differences in individuals belonging to the same species. Genetic diversity is generally low, ranging from 0.18 (D. callizonus) to 0.44 (D. henteri). Regarding the genetic diversity within populations of the same species, no differences were revealed by the use of the SSR markers tested in the present study.

Ključne riječi

Dianthus; endemic species; genetic structure; microsatellites; SSR markers; polymorphism

Hrčak ID:

206258

URI

https://hrcak.srce.hr/206258

Datum izdavanja:

1.10.2018.

Posjeta: 1.351 *