Veterinarska stanica, Vol. 51 No. 3, 2020.
Izvorni znanstveni članak
https://doi.org/10.46419/vs.51.3.6
Differentiation of alimentary lymphoma from inflammatory bowel disease and the influence of breed and age on the outbreak of pathological changes in the canine gastrointestinal tract
Aneta Piplica
; Veterinarski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Sven Menčik
; Veterinarski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Andrea Gudan Kurilj
; Veterinarski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Branka Artuković
; Veterinarski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Lidija Medven Zagradišnik
; Veterinarski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Marko Hohšteter
orcid.org/0000-0001-8844-2660
; Veterinarski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Sažetak
The aim of this study was to evaluate the use of immunohistochemistry (IHC) with the histopathological method to diagnose and differentiate alimentary lymphoma (AL) from inflammatory bowel disease (IBD). Archival histopathological findings and tissue samples, paraffin blocks and slides were used. This study included 35 dogs divided in three groups regarding breed size - small, medium or large - aiming to determine the correlation between certain pathohistological changes depending on breed, age and gender. The average age of dogs in the small breeds group was 7.58±3.59 years, in the medium breeds group was 7.45±3.04 years and in the large breeds group was 6.58±3.47 years. The average age of dogs suffering from AL was 8.8 years, unlike dogs suffering from IBD, with the average age of 7 years. A larger number of males was represented within all three groups. The highest share of biopsy samples (53.65%) involved the analysis of small intestine tissue, with eight samples (33.33%) in all three groups. The portion of the biopsied stomach tissue was somewhat lower (21.95%), while in two
breeds (4.87%) colon tissue was sampled. Histopathological analysis determined 16 cases of IBD, seven cases of lymphoma (20.00%), nine cases of suspected inflammation (25.71%) and three cases of suspected inflammation/lymphoma (8.58%). After IHC, the diagnosis determined by histopathological evaluation was confirmed in six cases, rejected in five cases, while in five cases it was useful in distinguishing AL from IBD. Significant differences (P<0.05) were found between the diagnosis of IBD and AL ascertained by pathohistological analysis and the IHC method. T cellular lymphoma was also found to be more common in the small intestine, while B cellular lymphoma was more prevalent in the stomach. A correlation (r) with the tendency of effect was recorded between the pathohistological diagnosis and the CD3 mark (r=-0.34; P=0.08), between the pathohistological diagnosis and the CD79 mark (r=0.36; P=0.09) with a slightly lower level of correlation between the pathohistological diagnosis and the diagnosis of IHC (r=0.21, P=0.34). Based on the results, it can be concluded that immunohistochemistry is useful in confirming the diagnosis and in the differentiation of AL and IBD.
Ključne riječi
dog; retrospective analysis; alimentary lymphoma; inflammatory bowel disease
Hrčak ID:
237808
URI
Datum izdavanja:
18.5.2020.
Posjeta: 2.643 *