Izvorni znanstveni članak
https://doi.org/10.33128/ag.81.4.5
THE USE OF A DIGITAL MICROSCOPE IN THE EVALUATION OF THE EFFECT OF HERBICIDE PENDIMETHALIN ON THE GROWTH OF BACTERIA Bradyrhizobium japonicum
Filipa Burul
; Agronomski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Ana Pintar
; Agronomski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Klara Barić
; Agronomski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Sažetak
The objective of this study was to determine the effect of different concentrations of herbicide pendimethalin on the growth of nitrogen-fixing bacteria Bradyrhizobium japonicum using a digital microscope. Pendimethalin was applied at concentrations of 0.00; 0.165; 0.33; 0.66; 1.32 and 2.64%, where the concentration of 0.66% presents the amount of herbicide which is usually applied in the field (recommended dose). The influence of pendimethalin on the growth of B. japonicum was determined by measuring the width of the inhibition zone around the discs 5, 7, 10 and 12 days after inoculation (DAI) of bacteria on a nutrient medium. The lowest inhibitory effect of pendimethalin for all concentrations determined was 5 DAI, when the average width of inhibition zone was between 0.18 mm for the lowest (0.165%) to 0.97 mm for the highest (2.64%) concentration. The progression of the inhibitory effect of pendimethalin occurred with every following assessment. The highest inhibitory effect determined was 12 DAI, when the width of the inhibiton zone at a concentration of 0.165% was 0.61 mm. The width of the inhibition zone significantly increased with increasing the pendimethalin concentrations and at a concentration of 2.64% it was 2.46 mm. The results of this study indicate the negative effect of herbicide pendimethalin on bacteria B. japonicum, even at concentrations lower than recommended concentrations that are applied in the field.
Ključne riječi
Bradyrhizobium japonicum; digital microscope; inhibition; pendimethalin
Hrčak ID:
239784
URI
Datum izdavanja:
18.5.2020.
Posjeta: 1.884 *