Izvorni znanstveni članak
Expression of Aspergillus oryzae Tannase in Pichia pastoris and Its Application in the Synthesis of Propyl Gallate in Organic Solvent
Xiao-Wei Yu
; Jiangnan University College of Biotechnology, CN-214122 Wuxi, PR China
Yong-Quan Li
; Zheijang University College of Life Science, CN-310058 Hangzhou, PR China
Sažetak
Gallic acid esters could be synthesized biologically by tannase in organic media, among which is propyl gallate, an antioxidant widely used as a food additive. Efficient intracellular expression of Aspergillus oryzae tannase was achieved in Pichia pastoris under the control of the AOX1 promoter, and the productivity of recombinant tannase was 960 U/L or 64 U/g dry mass. The recombinant P. pastoris was used to synthesize propyl gallate in organic solvent and the yield of propyl gallate was 53 %.
Ključne riječi
tannase; Pichia pastoris; recombinant protein production; propyl gallate; biosynthesis
Hrčak ID:
22226
URI
Datum izdavanja:
14.3.2008.
Posjeta: 2.976 *