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The extraction efficiency of maceration, UAE and MSPD in the extraction of pyrethrins from Dalmatian pyrethrum

Martina Grdiša orcid id orcid.org/ORCID ; University of Zagreb, Faculty of Agriculture, Department of Seed Science and Technology, Svetošimunska cesta 25, 10000 Zagreb;Centre of Excellence for Biodiversity and Molecular Plant Breeding (CoE CroP-BioDiv), Svetošimunska cesta 25, HR-10000 Zagreb
Filip Varga orcid id orcid.org/ORCID ; University of Zagreb, Faculty of Agriculture, Department of Seed Science and Technology, Svetošimunska cesta 25, 10000 Zagreb;Centre of Excellence for Biodiversity and Molecular Plant Breeding (CoE CroP-BioDiv), Svetošimunska cesta 25, HR-10000 Zagreb
Tonka Ninčević orcid id orcid.org/ORCID ; Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, 21000 Split
Barbara Ptiček orcid id orcid.org/ORCID ; student, University of Zagreb, Faculty of Agriculture
Dario Dabić orcid id orcid.org/ORCID ; University of Zagreb, Faculty of Chemical Engineering and Technology, Department of Analytical Chemistry, Marulićev trg 19, 10000 Zagreb
Martina Biošić orcid id orcid.org/ORCID ; University of Zagreb, Faculty of Chemical Engineering and Technology, Department of Analytical Chemistry, Marulićev trg 19, 10000 Zagreb


Puni tekst: engleski pdf 782 Kb

str. 257-267

preuzimanja: 189

citiraj


Sažetak

Dalmatian pyrethrum (Tanacetum cinerariifolium /Trevir./ Sch. Bip.) synthesizes secondary metabolite pyrethrin, known for its potent insecticidal and repellent activity. The present study was aimed at optimizing the maceration extraction parameters that improve the efficiency of pyrethrin extraction from the dried Dalmatian pyrethrum flower heads. Extraction efficiencies under several conditions were investigated: different solvent types, extraction time, the rotational speed of the stirrer, and the solvent volume. The highest recovery values were obtained with 5 mL of acetone, at the rotational speed of 400 rpm, and the extraction time of three hours. In addition, the extraction efficiency of maceration was compared to that of ultrasound-assisted and matrix solid phase dispersion extraction, both previously optimized for pyrethrin extraction. The extractions were carried out on samples of three natural Dalmatian pyrethrum populations (Krk, Mt. Kozjak, and Senj). Both the total pyrethrin content and the HPLC profile varied between different extraction techniques. Across applied methods, the highest efficiency was observed with matrix solid phase dispersion extraction. Evaluation of the differences between data obtained using different extraction techniques was performed by the Bland-Altman analysis, revealing good agreement between the three methods.

Ključne riječi

Dalmatian pyrethrum, MSPD, UAE, maceration, optimization, pyrethrin, Tanacetum cinerariifolium

Hrčak ID:

243611

URI

https://hrcak.srce.hr/243611

Posjeta: 365 *