Medicina Fluminensis, Vol. 57 No. 3, 2021.
Stručni rad
https://doi.org/10.21860/medflum2021_261191
Antioxidative synergism of the squeezed aronia juice and red wine mixtures of three grape varieties from the Bujština area
Ela Mijatović
orcid.org/0000-0001-6237-1243
; Sveučilište u Rijeci, Medicinski fakultet, Rijeka, Hrvatska
Dalibor Broznić
orcid.org/0000-0003-3935-5338
; Zavod za medicinsku kemiju, biokemiju i kliničku kemiju, Sveučilište u Rijeci, Medicinski fakultet, Rijeka, Hrvatska
Sažetak
Aim: To determine the synergism of antioxidants from natural samples of chokeberry juice and red wine as well as to examine the reaction kinetics between DPPH radical and antioxidants presented in the samples. Materials and Methods: Antioxidant activity in five methanolic samples (ARONIJA, VINO, MIX 1 (50% chokeberry juice and 50% red wine), MIX 2 (75% chokeberry juice and 25% red wine), MIX 3 (25% chokeberry juice and 75% red wine)) was measured spectrophotometrically using DPPH• and TEAC assays, while the chemical reaction of kinetics was analyzed with three monophasic and one biphasic mathematical models. Results: TEAC test shows the best antioxidants synergism of, as well as the strongest antioxidant capacity for mixture, MIX 1 (75 mmol/kg), followed by MIX 3 and MIX 2 (67 and 62 mmol/kg). The inhibition of DPPH radical signal was the strongest in the ARONIJA sample (81%), while it was the weakest in the VINO sample (21%). By combining beverages, two different types of antioxidants inhibited the DPPH radical causing two phases of reaction with different chemical reaction rates. Conclusion: The addition of chokeberry juice to red wine causes an increase in its antioxidant activity. Furthermore, if the chokeberry juice and red wine mixture is prepared, the samples should be in equal amount (50:50) in order to achieve the best antioxidants synergism and also strengthened the health effect.
Ključne riječi
antioxidants; chokeberry; DPPH radical; kinetics; TEAC assay; wine
Hrčak ID:
261191
URI
Datum izdavanja:
1.9.2021.
Posjeta: 1.634 *