Veterinarski arhiv, Vol. 77 No. 2, 2007.
Izvorni znanstveni članak
Characterization of leptospiral isolates using random amplified polymorphic DNA (RAPD) fingerprinting
Isam El Jalii
orcid.org/0000-0002-5793-5873
; Department of Preventive Medicine and Public Health, Faculty of Veterinary Medicine, University of Khartoum, Khartoum North, Sudan
Abdul Rani Bahaman
; Faculty of Veterinary Medicine, University Putra Malaysia, Serdang, Selangor, Malaysia
Sažetak
Random amplified polymorphic DNA (RAPD) fingerprinting was applied to characterize leptospiral strains. Two primers were used to generate individual RAPD fingerprints. The DNA fingerprints obtained were distinct and reproducible. The fingerprints obtained could be useful for distinguishing and characterizing the strains. Profiles obtained revealed genetic heterogeneity between strains belonging to one serovar. In conclusion, different DNA fingerprints were obtained which would allow characterization of the strains.
Ključne riječi
RAPD; leptospira; serovar; characterization
Hrčak ID:
25094
URI
Datum izdavanja:
20.4.2007.
Posjeta: 1.043 *