Stručni rad
https://doi.org/10.33128/ag.83.3.1
Occurrence and distribution of economically important viruses of zucchini (Cucurbita pepo L.) and pumpkin (Cucurbita sp.) in Croatia
Dario Ivić
; Hrvatska agencija za poljoprivredu i hranu, Centar za zaštitu bilja, Zagreb, Hrvatska
Jasna Milanović
; Hrvatska agencija za poljoprivredu i hranu, Centar za zaštitu bilja, Zagreb, Hrvatska
Lea Mihaljevski Boltek
; Hrvatska agencija za poljoprivredu i hranu, Centar za zaštitu bilja, Zagreb, Hrvatska
Adrijana Novak
; Hrvatska agencija za poljoprivredu i hranu, Centar za zaštitu bilja, Zagreb, Hrvatska
Darko Vončina
; Agronomski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Sažetak
Viral diseases of zucchini and pumpkin have become more severe in recent years, causing significant yield losses. The objective of this study was to determine the presence of cucumber mosaic virus (CMV), zucchini yellow mosaic virus (ZYMV) and watermelon mosaic virus (WMV) in these crops. In 2019, a survey was conducted at 18 sites (open fields) in six counties. Fortythree symptomatic samples of zucchini and 39 symptomatic samples of pumpkin were collected and tested for the presence of CMV, ZYMV and WMV. Samples were tested with commercial serological assays, using doubleantibody sandwich ELISA kits. Out of 82 samples analysed, CMV was detected in 55 (67%), ZYMV in 50 (61%), and WMV in 10 samples (12%). Single viral infection was detected in 38 samples (46%). Mixed infections with two viruses were recorded in 36 samples (44%), CMV + ZYMV combination in 32 samples (39%), and CMV + WMV combination in four samples (5%). Triple viral infection was detected in two samples, while six samples (7%) were determined to be free of all three viruses in the testes. One virus species was recorded at eight locations, two viruses at nine locations, while all three viruses were determined in one out of 18 sampled fields.
Ključne riječi
Cucurbita pepo L..; cucumber mosaic virus (CMV); zucchini yellow mosaic virus (ZYMV); watermelon mosaic virus (WMV); ELISA
Hrčak ID:
275612
URI
Datum izdavanja:
21.4.2022.
Posjeta: 718 *