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MOLECULAR TYPING OF TUNISIAN CLONES OF Myzus persicae (HEMIPTERA: APHIDIDAE) USING MICROSATELLITE MARKERS

Fattouma Djilani Khouadja ; Laboratoire de Genetique Moleculaire, Immunologie et Biotechnologie, Faculte des Sciences de Tunis, Tunis, Tunisie
Joelle Rouze Jouan ; Laboratoire de Zoologie UMR BiO3P, INRA Le Rheu, Rennes Cedex, France
Jean Christophe Simon ; Laboratoire de Zoologie UMR BiO3P, INRA Le Rheu, Rennes Cedex, France
Nathalie Leterme ; Laboratoire de Zoologie UMR BiO3P, INRA Le Rheu, Rennes Cedex, France
Sonia Bou Hachem ; Institut National de la Recherche Agronomique de Tunisie
Mohamed Marrakchi ; Laboratoire de Genetique Moleculaire, Immunologie et Biotechnologie, Faculte des Sciences de Tunis, Tunis, Tunisie
Hatem Fakhfakh ; Laboratoire de Genetique Moleculaire, Immunologie et Biotechnologie, Faculte des Sciences de Tunis, Tunis, Tunisie

Sažetak

In order to assess the genetic differentiation among Tunisian clones belonging to the Myzus persicae complex (M. persicae (Sulzer), M. antirrhinii (Macchiati) and M. nicotianea Blackman), the molecular technique of microsatellites was used in this study. These markers offer sensitivity and are useful in population genetic studies of parthenogenetic organisms. Here, nine polymorphic microsatellite loci were amplified to distinguish between six parthenogenetic clones belonging to M. persicae complex collected from two different Tunisian areas. Interestingly, this technique allowed discrimination between five different genotypic classes among the six clones. Furthermore, analysis of genetic relatedness between the genotypic classes revealed that two Tunisian clones did not cluster either in M. persicae or in M. antirrhinii taxa, whereas, the four other Tunisian clones clustered into the M. persicae Sulzer taxa.

Ključne riječi

Hemiptera; Aphididae; Myzus persicae complex; clones; molecular typing; parthenogenesis; microsatellite markers; Tunisie

Hrčak ID:

283853

URI

https://hrcak.srce.hr/283853

Datum izdavanja:

31.1.2004.

Podaci na drugim jezicima: hrvatski

Posjeta: 294 *