Entomologia Croatica, Vol. 5 No. 1-2, 2001.
Izvorni znanstveni članak
DISTINCTION OF TWO SPECIES OF CECIDOMYIES (DIPTERA: CECIDOMYIIDAE) REVEALED BY MITOCHONDRIAL DNA GENES VARIATION
Maha Mezghani Khemakhem
; Laboratoire de Genetique Moleculaire, Immunologie et Biotechnologie, Faculte des Sciences de Tunis, Tunis, Tunisie
Hanem Makni
; Laboratoire de Genetique Moleculaire, Immunologie et Biotechnologie, Faculte des Sciences de Tunis, Tunis, Tunisie
Mohamed Marrakchi
; Laboratoire de Genetique Moleculaire, Immunologie et Biotechnologie, Faculte des Sciences de Tunis, Tunis, Tunisie
Sažetak
Mayetiola is an important pest of cereal in Tunisia. Two species were identified using isoenzymatic markers. To develop a DNA diagnostic tool, we assessed mitochondrial DNA study based on Restriction Fragment Length Polymorphism in Polymerase Chain Reaction Amplified Fragments (PCR -RFLP). Specific primers were used to amplify the cytochrome b and a 3’ terminal region of the 12S mt rDNA genes. Results showed that Sau3A restriction patterns can distinguish between the two morphologically similar species. Thus PCR-RFLP is a useful tool for identifying morphologically simiIar species of Mayetiola in large-scale pest identification.
Ključne riječi
Mitochondrial DNA; PCR-RFLP; Diptera; Cecidomyiidae; Mayetiola; Tunisie
Hrčak ID:
284078
URI
Datum izdavanja:
31.1.2002.
Posjeta: 266 *