Original scientific paper

https://doi.org/10.20471/acc.2022.61.02.04

Clinical Application of RT-PCR in Tuberculosis DNA Detection Combined with TB-IGRA in the Diagnosis of Sputum Smear-Negative Pulmonary Tuberculosis

Gao Chen ; Department of Infectious Diseases, Deyang People’s Hospital, Deyang Sichuan, People’s Republic of China
Chun-jun Qin ; Department of Infectious Diseases, Deyang People’s Hospital, Deyang Sichuan, People’s Republic of China
Meng-zheng Wu ; Department of Infectious Diseases, Deyang People’s Hospital, Deyang Sichuan, People’s Republic of China
Bi-Bo Wu ; Department of Infectious Diseases, Deyang People’s Hospital, Deyang Sichuan, People’s Republic of China
Wan-Rong Luo ; Department of Infectious Diseases, Deyang People’s Hospital, Deyang Sichuan, People’s Republic of China
Han Zhuang ; Department of Infectious Diseases, Deyang People’s Hospital, Deyang Sichuan, People’s Republic of China
Xian-ya He ; Department of Infectious Diseases, Deyang People’s Hospital, Deyang Sichuan, People’s Republic of China
Shu-Shu Liu ; Department of Infectious Diseases, Deyang People’s Hospital, Deyang Sichuan, People’s Republic of China

Abstract

The aim was to investigate detection of pulmonary alveolar lavage fluid tuberculosis
DNA by real-time fluorescent polymerase chain reaction (RT-PCR) combined with clinical application
of the sputum smear-negative pulmonary tuberculosis diagnosis with TB interferon-γ release
assay (TB-IGRA). From October 2014 to October 2015, 632 outpatients and inpatients treated in our
hospital were randomly selected, of which 459 patients as the research group managed with RT-PCR
detection combined with TB-IGRA and 173 patients as the control group undergoing electronic
bronchoscopy alveolar lavage fluid detection, with detection results statistically evaluated. The positive
rate in the research group was 96.51%, i.e. significantly higher than that in the control group (66.47%),
yielding a statistically significant difference (χ2=109.68, p=0.00). The true positive rate was 97.7% in
the research group and 67.92% in the control group; the true positive rate was significantly higher in
the research group patients as compared with the control group, yielding a statistically significant difference
(χ2=112.04, p=0.00). The sensitivity and specificity, as well as Youden index were significantly
higher in the research group as compared with the control group. In conclusion, TB DNA detection
by RT-PCR combined with TB-IGRA is a very good method of diagnosing tuberculosis, and it can
be implemented in clinical diagnosis of pulmonary tuberculosis.

Keywords

RT-PCR; TB-IGRA; Alveolar lavage fluid; Diagnosis

Hrčak ID:

284671

URI

https://hrcak.srce.hr/284671

Publication date:

1.8.2022.

Article data in other languages: croatian

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