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Veterinarska stanica, Vol. 56 No. 1, 2025.

Izvorni znanstveni članak

https://doi.org/10.46419/vs.56.1.11

First report on the presence of potentially pathogenic Vibrio parahaemolyticus in farmed mussels (Mytilus galloprovincialis) from the Istrian aquatorium by microbiological and molecular methods

Natalija Džafić orcid id orcid.org/0000-0001-7658-5517 ; Croatian Veterinary Institute, Branch Veterinary Institute Rijeka, Croatia
Barbara Boljkovac Begić orcid id orcid.org/0009-0000-1423-0075 ; Croatian Veterinary Institute, Branch Veterinary Institute Rijeka, Croatia
Karmela Nina Barać orcid id orcid.org/0009-0003-9474-6363 ; Croatian Veterinary Institute, Branch Veterinary Institute Rijeka, Croatia
Miroslav Benić ; Croatian Veterinary Institute, Zagreb, Croatia
Sanja Duvnjak ; Croatian Veterinary Institute, Zagreb, Croatia
Lidija Kozačinski ; Faculty of Veterinary Medicine University of Zagreb, Croatia
Kristina Kvrgić orcid id orcid.org/0000-0002-2102-1113 ; Croatian Veterinary Institute, Branch Veterinary Institute Rijeka, Croatia *
Andrea Humski orcid id orcid.org/0000-0003-3027-1306 ; Croatian Veterinary Institute, Zagreb, Croatia

* Dopisni autor.

Puni tekst: engleski pdf 1.459 Kb

verzije

str. 1-12

preuzimanja: 149

citiraj

Sažetak

Microbiological and molecular PCR techniques were used to analyse 328 samples of mussels (Mytilus galloprovincialis) cultured in Istrian waters. Molecular testing was more effective than microbiological testing, detecting the presence of the bacterium Vibrio parahaemolyticus in 21.34% of samples, compared to 2.44% detected through microbiological methods. The probability of detecting the presence of this bacterium was 7.19 times higher when the sea surface temperature was above 15°C, while thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) positive samples were detected at sea temperatures above 22.5°C and 24.7°C, respectively. TDH- and TRH-positive samples constituted 10% of the total V. parahaemolyticus-positive samples. As there is no legal obligation to monitor the presence of this bacterium, consuming raw or insufficiently cooked mussels can lead to illness.

Ključne riječi

mussels; Vibrio parahaemolyticus; microbiology; PCR

Hrčak ID:

317596

URI

https://hrcak.srce.hr/317596

Datum izdavanja:

11.6.2024.

Podaci na drugim jezicima: hrvatski

Posjeta: 509 *