Sjemenarstvo, Vol. 35 No. 1, 2024.
Pregledni rad
The strategies in the detection of genetically modified organisms in the seed on the territory of the Republic of Croatia: the application of the screening method
Ksenija Duka
; Hrvatska agencija za poljoprivredu i hranu, Centar za sjemenarstvo i rasadničarstvo, Usorska 19, Brijest, Hrvatska
Renata Hanzer
; Hrvatska agencija za poljoprivredu i hranu, Centar za sjemenarstvo i rasadničarstvo, Usorska 19, Brijest, Hrvatska
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* Autor za dopisivanje.
Sažetak
With the market placement of the first genetically modified organisms in the 1990s, there was a necessity for appropriate analytical methods of their monitoring. Modern biotechnology is among the most rapidly developed present‐day technologies, which is why every year there is an increase in new modifications on the market, and, as a result, a number of methods for their detection also increases. In the initial years of monitoring the genetically modified organisms, the analysis involved determining the presence of only 2-3 screening elements, while today the laboratories have to cover over a hundred different modifications. An increasing number of methods presents a challenge to the laboratories due to an increase in the number of analytical steps that are necessary to be performed per sample, analyses are becoming more time‐consuming and financially demanding, and a successful strategy in analytics is necessary. In Croatia, the genetically modified crops are systematically monitored through national monitoring. The monitoring involves the analyses of seeds, green leaves, food, and animal feed. Considering a constant increase in market modifications, an increasing number of analyses are annually performed on the same number of samples. This paper investigates the application of matrices for the selection of screening methods in accordance with the monitoring plan. The screening methods precede the confirmatory Real Time PCR analyses and serve to exclude the negative samples. Given that the analytical procedures must include all currently approved modifications, a screening matrix was used to achieve a maximum coverage of all modifications with as few analytical steps as possible and ensure a purposeful analysis. The research results demonstrate that the screening matrix enables an optimal selection of strategy in the sample analysis, produces a satisfactory result, and, simultaneously, the analyses and the financial cost are within acceptable limits.
Ključne riječi
screening methods; GMO; matrix; monitoring; Real Time PCR
Hrčak ID:
319774
URI
Datum izdavanja:
25.7.2024.
Posjeta: 0 *