Izvorni znanstveni članak
https://doi.org/10.20471/LO.2024.52.02-03.08
Morphometric objectification in determining the grade of invasive breast cancer
Ivana Prvulović
; Polyclinic Prvulovic, Slavonski Brod, Croatia
Vesna Ramljak
; Department of Clinical Cytology, University Hospital for Tumors, Ljudevit Jurak University Department of Pathology and Cytology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Mirjana Stupnišek
orcid.org/0000-0003-2109-2787
; University Hospital for Infectious Diseases, Zagreb, Croatia and Faculty of Dental Medicine and Health, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Damir Jurić
; University Hospital for Infectious Diseases, Zagreb, Croatia
Valerija Miličić
orcid.org/0009-0001-2205-9287
; University Hospital for Infectious Diseases, Zagreb, Croatia and Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia
Sažetak
Objective: The study aimed to apply morphometric grading to cytological samples of invasive breast cancer and assess the potential for routine application, to describe the biological variability of morphometric parameters within and between grades, to optimize and determine the minimal, currently undefined, sample size required for morphometric analysis.
Subjects and methods: The study included 45 female patients diagnosed with breast cancer on cytological breast aspirates and confirmed on histological samples at the General Hospital Dr. Josip Benčević. Morphometric analysis was conducted using the SFORM software.
Results: The analysis of differences in the distribution of morphometric parameters by grade shows a significant, gradual increase in measured features with increasing grade. Grade accounts for approximately 35% of the variability in cell and nucleus morphometric characteristics. The majority of the variability in characteristics results from interindividual differences or heterogeneity of grade among participants, with minimal intraindividual variations. The minimal, currently undefined, sample size required for morphometric analysis was standardized to 100 cells per sample, as this ensures a narrow margin of error.
Conclusion: Grading by the morphometric method is statistically significant, but due to the low contribution of the grade to the variability of the morphometric characteristics, and the wide range of overlapping morphometric parameters among the grades, morphometry as a method does not have discriminatory but only indicative capability.
Ključne riječi
breast cancer; fine needle aspiration cytology; morphometry
Hrčak ID:
328484
URI
Datum izdavanja:
6.3.2025.
Posjeta: 601 *