Veterinarski arhiv, Vol. 95 No. 4, 2025.
Izvorni znanstveni članak
https://doi.org/10.24099/vet.arhiv.2633
Učinak nesfatina 1 na autofagiju moždanog tkiva i lučenje dopamina u miševa nakon gladovanja
Siriguleng Yu
; College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology for Animal Diseases, Ministry of Agriculture, Hohhot, China
*
Xin Wen
; College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology for Animal Diseases, Ministry of Agriculture, Hohhot, China
Dong Ai
; Agriculture and Animal Husbandry Bureau of Alxa League, Alxa league, China
Yuchen Luo
; College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology for Animal Diseases, Ministry of Agriculture, Hohhot, China
Ziyi Li
; College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology for Animal Diseases, Ministry of Agriculture, Hohhot, China
Haoyu Bai
; College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology for Animal Diseases, Ministry of Agriculture, Hohhot, China
Wen Yu
; College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology for Animal Diseases, Ministry of Agriculture, Hohhot, China
Xingchuan He He
; College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology for Animal Diseases, Ministry of Agriculture, Hohhot, China
Yaru Niu
; College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology for Animal Diseases, Ministry of Agriculture, Hohhot, China
Junjian Jin
; College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology for Animal Diseases, Ministry of Agriculture, Hohhot, China
Shumin Du
; College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology for Animal Diseases, Ministry of Agriculture, Hohhot, China
* Dopisni autor.
Sažetak
Poznato je da povremeno gladovanje (engl. intermittent fasting) utječe na metaboličke i neuroendokrine puteve a nesfatin 1, peptid koji sudjeluje u regulaciji apetita i metabolizmu, može biti ključan u tim procesima. U radu je istražen utjecaj nesfatina 1 na autofagiju moždanog tkiva i lučenje dopamina u miševa nakon gladovanja. Četrdeset C57BL/6 miševa nasumično je podijeljeno u kontrolnu skupinu, skupinu miševa koji su gladovali 24 sata, skupinu miševa koji su gladovali 48 sati, skupinu miševa nesfatin 1 + 24 h gladovanja i skupinu miševa nesfatin 1+ 48 h gladovanja. Na- kon intraperitonealnih injekcija od 1,25 nmol/g nesfatina 1, primijenjenog poslije odgovarajućeg razdoblja gladovanja, određene su serumske razine nesfatina 1 i dopamina, kao i izražaj mRNA i proteina tirozin-hidroksilaze te čimbenika autofagije u moždanim tkivima. Pri navedenom korištene su metode ELISA, PCR u stvarnom vremenu (qPCR) i west- ern blot. U skupinama miševa koji su gladovali rezultati su pokazali znakovito sniženje serumskih razina nesfatina 1 i dopamina (P<0,05) u usporedbi s kontrolnom skupinom, zajedno s povećanim izražajem mRNA i proteina Beclin 1 te lakog lanca 3 proteina 1 povezanog s mikrotubulima u moždanim tkivima. Također u skupinama miševa koji su gladova- li utvrđena je snižena razina p62 i tirozin - hidroksilaze (P<0,01). Dodatak nesfatina 1 doveo je, međutim, do znakovitog porasta serumskih razina nesfatina 1 i dopamina (P<0,01), kao i smanjenja izražaja proteina Beclin 1 i lakog lanca 3 proteina 1(P<0,01) te porasta razine p62 i tirozin-hidroksilaze (P<0,01). Ovi rezultati pokazuju da u miševa produljeno gladovanje snižava razine nesfatina 1 i dopamina te povećava autofagiju moždanog tkiva, dok dodatak nesfatina 1 ne samo da učinkovito smanjuje autofagiju u mozgu već i potiče lučenje dopamina.
Ključne riječi
autofagija; dopamine; post; nesfatin 1; tirozin-hidroksilaza
Hrčak ID:
337475
URI
Datum izdavanja:
1.10.2025.
Posjeta: 0 *