Izvorni znanstveni članak
https://doi.org/10.5513/JCEA01/27.1.4927
Occurrence and diversity of apple mosaic ilarvirus in apple and hazelnut in Serbia
Bojana VASILIJEVIĆ
; Fruit Research Institute, Kralja Petra I nr. 9, 32102 Čačak, Serbia
*
Vera KATANIĆ
; Fruit Research Institute, Kralja Petra I nr. 9, 32102 Čačak, Serbia
Darko JEVREMOVIĆ
; Fruit Research Institute, Kralja Petra I nr. 9, 32102 Čačak, Serbia
* Dopisni autor.
Sažetak
Two hundred and twenty trees (150 apples and 70 hazelnuts) from seven locations in Serbia were tested for the presence of Ilarvirus ApMV (apple mosaic virus). ELISA test confirmed the presence of ApMV in 11 apples (7.3%) and six hazelnuts (8.5%) samples, with typical and severe mosaic symptoms on the leaves. ELISA-positive samples were further tested by RT-PCR with two different primer pairs. One primer pair was efficient for the RT-PCR detection of ApMV isolates from apple, and the other one for isolates from hazelnut. Sequence analysis of amplified PCR fragments of the selected isolates confirmed that Serbian ApMV isolates from apple share the highest nucleotide identity (98.66 ‒ 99.02%) with isolates from India and Canada.
Ključne riječi
Malus × domestica Borkh.; Corylus avellana L.; double antibody sandwich enzyme-linked immunosorbent assay; reverse transcription polymerase chain reaction; sequencing
Hrčak ID:
346061
URI
Datum izdavanja:
7.4.2026.
Posjeta: 0 *