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Characterization of Glucose Oxidase from Penicillium notatum

Haq Nawaz Bhatti ; Industrial Biotechnology Laboratory, Department of Chemistry and Biochemistry, University of Agriculture, PK-38040 Faisalabad, Pakistan
Nabeela Saleem ; Industrial Biotechnology Laboratory, Department of Chemistry and Biochemistry, University of Agriculture, PK-38040 Faisalabad, Pakistan

Sažetak

In the present study glucose oxidase (GOD) has been isolated from a culture filtrate of Penicillium notatum. The enzyme was purified by ammonium sulphate precipitation, diethylaminoethyl (DEAE) cellulose ion-exchange chromatography and Sephadex gel filtration. This protocol gave 16.47-fold purification and 25 % recovery of the enzyme. The optimum pH and temperature for the activity were 5.4 and 45 °C, respectively. The Km and vmax values for the enzyme were 10.5 mM and 456 U/mg, respectively. A detailed kinetic study of thermal inactivation was carried out. Both enthalpy of activation (ΔH*) and entropy of activation (ΔS*) decreased at higher temperatures. Moreover, free energy of denaturation (ΔG*) increased at higher temperature, making the enzyme thermally stable. A possible explanation for the thermal inactivation of GOD at higher temperatures is also discussed.

Ključne riječi

Penicillium notatum; glucose oxidase; thermostability; purification

Hrčak ID:

39851

URI

https://hrcak.srce.hr/39851

Datum izdavanja:

7.8.2009.

Podaci na drugim jezicima: hrvatski

Posjeta: 2.588 *