Izvorni znanstveni članak
Association of Polymorphism of the MBL2 Gene in Type 2 Diabetic Patients with Periodontitis: A Preliminary Study
Natalia COSTA ARAUJO
; Federalno Sveučilište Pernambuco, Recife, Pernambuco, Brazil
Darcyla Maria de AGUIAR BELLO
; Federalno Sveučilište Pernambuco, Recife, Pernambuco, Brazil
Paulo Roberto Eleutério de SOUZA
; Zavod za biologiju Federalnog Sveučilišta Pernambuco, Recife, Pernambuco, Brazil
Renata Cimões
orcid.org/0000-0003-3673-8739
; Zavod za stomatološku protetiku i oralnu i facijalnu kirurgiju, Stomatološki fakultet Federalnog Sveučilišta Pernambuco, Recife, Pernambuco, Brazil
Sažetak
Aim: Mannose-binding lectin (MBL) is an important protein of the innate immune system. It is able to bind to sugar groups encountered on the surfaces of a wide range of microorganisms and interacts with serine proteases (MASPs) to effect the activation of complement. Mutations of the expressed human MBL2 gene have been associated with several infections; this preliminary study thus sets out to assess the association between the polymorphism in exon-1 of the MBL2 gene and periodontal disease in type 2 diabetic patients. Material and Methods: The sample comprised 50 diabetic patients, who were submitted to a clinical periodontal examination that evaluated in six sites per tooth probing depth, bleeding on probing, clinical recession, dental plaque and the number of teeth present. Periodontal disease was defined as 4+ sites of loss of attachment of 5+ mm, with one or more of those sites having a pocket of 4+ mm (Beck’s criteria). The collection of scaling cells from the oral mucosa was carried out and the detection of polymorphism was made by the technique of real time PCR (RT-PCR) using the melting temperature curve analysis. Results: The data revealed that no statistically significant differences in the genotype (p=0.564) or the allele (p=0.643) frequencies were observed among the healthy individuals or those with periodontitis. Conclusions: The polymorphism in exon-1 of the MBL2 gene was not related to the periodontal disease in the sample evaluated.
Ključne riječi
Mannose-Binding Lectin; Polymorphism, Genetic; Periodontitis; Diabetes Mellitus, Type 2
Hrčak ID:
45302
URI
Datum izdavanja:
15.12.2009.
Posjeta: 2.555 *