Izvorni znanstveni članak
Purification, composition and antioxidant activity of polysaccharides from wolfberry, cherry, kiwi and cranberry fruits
Huiping Fan
; Henan Agricultural University, College of Food Science and Technology, No.95 Wenhua Road, Jinshui District, Zhengzhou, Henan 450002, China
G. Mazza
; Agriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, 4200 Highway 97, P.O. Box 5000 Summerland, British Columbia V0H 1Z0, Canada
X. Liao
; China Agricultural University, College of Food Science & Nutritional Engineering, Key Laboratory of Fruit & Vegetable Processing, No. 17 Qinghua East Road, P.O. Box 303 Haidian District, Beijing 100083, China
Sažetak
Water-soluble polysaccharides from wolfberry (Lycium barbarum L.), sweet cherry (Prunus avium L.), kiwi (Actinidia chinensis L.) and cranberry fruits (Vaccinium macrocarpon Aiton) were extracted with boiling water, fractionated using ion exchange column chromatography, and characterized for molecular weight by high performance size exclusion chromatography (HPSEC). Monomer sugar composition was determined by gas chromatography (GC), and antioxidant activity was assayed by oxygen radical absorbance capacity (ORAC) and Trolox equivalent antioxidant capacity (TEAC). All four types of fruit investigated had four separate polysaccharide fractions; however, the polysaccharides from sweet cherries had higher molecular weight fractions. All the fruits contained rhamnose, fucose, arabinose, xylose, mannose, galactose, and glucose, but the polysaccharides from different fruits, and from cherries of different cultivars and maturity levels, had different ratios of simple sugars. TEAC and ORAC assays revealed that raw and purified polysaccharides from cherries, cranberries, kiwi, and wolfberries have antioxidant activity, and sweet cherry polysaccharides have the highest antioxidant activity.
Ključne riječi
Antioxidant activity; molecular weight; purification; polysaccharides; sugar composition
Hrčak ID:
59549
URI
Datum izdavanja:
16.7.2010.
Posjeta: 5.169 *