Original scientific paper
Occurrence of Killer Yeast Strains in Fruit and Berry Wine Yeast Populations
Gintare Gulbiniene
; Laboratory of Genetics, Institute of Botany, Zaliuju ezeru 49, LT-08406 Vilnius, Lithuania
Laima Kondratiene
; Laboratory of Genetics, Institute of Botany, Zaliuju ezeru 49, LT-08406 Vilnius, Lithuania
Tautvile Jokantaite
; Laboratory of Genetics, Institute of Botany, Zaliuju ezeru 49, LT-08406 Vilnius, Lithuania
Elena Serviene
; Laboratory of Genetics, Institute of Botany, Zaliuju ezeru 49, LT-08406 Vilnius, Lithuania
Vytautas Melvydas
; Laboratory of Genetics, Institute of Botany, Zaliuju ezeru 49, LT-08406 Vilnius, Lithuania
Giedre Petkuniene
; Department of Gene Engineering, Institute of Biochemistry, Mokslininku 12, LT-08662 Vilnius, Lithuania
Abstract
Apple, cranberry, chokeberry and Lithuanian red grape wine yeast populations were used for the determination of killer yeast occurrence. According to the tests of the killer characteristics and immunity the isolated strains were divided into seven groups. In this work the activity of killer toxins purified from some typical strains was evaluated. The analysed strains produced different amounts of active killer toxin and some of them possessed new industrially significant killer properties. Total dsRNA extractions in 11 killer strains of yeast isolated from spontaneous fermentations revealed that the molecular basis of the killer phenomenon was not only dsRNAs, but also unidentified genetic determinants.
Keywords
dsRNA; immunity; killer activity; Saccharomyces cerevisiae; wine yeast
Hrčak ID:
110837
URI
Publication date:
15.9.2004.
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