Short communication, Note
Streptomyces rimosus GDS(L) Lipase: Production, Heterologous Overexpression and Structure-Stability Relationship
Dušica Vujaklija
; Ruđer Bošković Institute, Department of Molecular Genetics, Department of Organic Chemistry and Biochemistry, Department of Physical Chemistry, Bijenička cesta 54, HR-10002 Zagreb, Croatia
Marija Abramić
; Ruđer Bošković Institute, Department of Molecular Genetics, Department of Organic Chemistry and Biochemistry, Department of Physical Chemistry, Bijenička cesta 54, HR-10002 Zagreb, Croatia
Ivana Leščić
; Ruđer Bošković Institute, Department of Molecular Genetics, Department of Organic Chemistry and Biochemistry, Department of Physical Chemistry, Bijenička cesta 54, HR-10002 Zagreb, Croatia
Tereza Maršić
; PLIVA Research Institute, Therapeutic Area 2, Prilaz Baruna Filipovića 25, HR-10000 Zagreb, Croatia
Jasenka Pigac
; Ruđer Bošković Institute, Department of Molecular Genetics, Department of Organic Chemistry and Biochemistry, Department of Physical Chemistry, Bijenička cesta 54, HR-10002 Zagreb, Croatia
Abstract
Streptomyces rimosus lipase gene has been overexpressed in a heterologous host, S. lividans TK23. The maximal lipase activity was determined in the culture filtrates of the late stationary phase. Time course of lipase production was monitored by a modified plate assay. S. rimosus lipase gene has been located on the AseI B fragment approximately 2 Mb far from the left end of the S. rimosus linear chromosome. Out of eight examined streptomycetes, the presence of this rare type of bacterial lipase gene was detected in two belonging to the S. rimosus taxonomic cluster, and in one non-related species. Comparison of protein sequences of the Streptomyces lipolytic enzymes was performed. The result indicated the best structural stability of the putative S. coelicolor lipase-2.
Keywords
lipase; Streptomyces; plate assay; thermostability
Hrčak ID:
111175
URI
Publication date:
17.3.2003.
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