Original scientific paper

Cultivable Bacteria from Milk from Slovenian Breastfeeding Mothers

Tina Tušar ; Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia
Klavdija Žerdoner ; Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia
Bojana Bogovič Matijašić orcid.org/0000-0003-0666-6981 ; Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia
Diana Paveljšek ; Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia
Evgen Benedik orcid.org/0000-0002-6954-1118 ; Department of Gastroenterology, Hepatology and Nutrition, University Children’s Hospital Ljubljana, University Medical Centre Ljubljana, Bohoričeva 20, SI-1000 Ljubljana, Slovenia
Borut Bratanič ; Department of Neonatology, University Children’s Hospital Ljubljana, University Medical Centre Ljubljana, Bohoričeva 20, SI-1000 Ljubljana, Slovenia
Nataša Fidler ; Department of Gastroenterology, Hepatology and Nutrition, University Children’s Hospital Ljubljana, University Medical Centre Ljubljana, Bohoričeva 20, SI-1000 Ljubljana, Slovenia
Irena Rogelj orcid.org/0000-0002-2056-4888 ; Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230 Domžale, Slovenia

Abstract

The human milk microbiota plays an important role in the development of infant´s intestinal microbiota and in the protection of infants against pathogenic microorganisms. The aim of this study is to investigate the microbial composition of human milk from 47 breastfeeding mothers, sampled separately from the left (L) and the right (R) breast, on the 30th day after giving birth. We quantified some major bacterial groups in human milk, compared the cultivable bacteria from the left and the right breast and identified strain diversity of lactobacilli. The results revealed that human milk contains lactic acid bacteria, bifidobacteria and mesophilic aerobic bacteria, of which the last were the most abundant group. Although the microbial composition of human milk in L and R breast samples was comparable, the concentration of bacteria in the two samples from the same mother might vary, therefore milk sample taken from one breast only does not reflect the average microbial composition. Using random amplified polymorphic DNA (RAPD), 86 presumptive isolates of lactobacilli from representative samples of human milk from 11 mothers were classified into 11 groups. Moreover, representatives of different RAPD groups were identified using 16S rDNA sequencing. Out of 11 RAPD groups, 4 groups (21 % of all isolates) belonged to the species Lactobacillus gasseri. The most representative RAPD profile (48 % of isolates) was found to belong to the species Lactobacillus fermentum. Other RAPD groups were associated with L. salivarius, L. reuteri, Enterococcus faecium, Staphylococcus epidermidis and Bifidobacterium breve species.

Keywords

human milk; cultivable microbiota; RAPD PCR; Lactobacillus sp.; 16S rDNA sequencing

Hrčak ID:

122378

URI

https://hrcak.srce.hr/122378

Publication date:

15.6.2014.

Article data in other languages: croatian

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