Preliminary communication

https://doi.org/10.18047/poljo.21.1.sup.21

THE PHYSICAL FORM OF CORN INFLUENCES THE RUMEN BACTERIAL BIODIVERSITY – PRELIMINARY RESULTS

Giulia Rossi ; Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Legnaro (PD), Italy
Franco Tagliapietra ; Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Legnaro (PD), Italy
Eric Pinloche ; Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, SY23 3AL, United Kingdom
Gabriel De La Fuente ; Department of Animal Production, University of Lleida, Alcalde Rovira Roure 191, 25198 Lleida, Spain
Stefano Schiavon ; Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Legnaro (PD), Italy
Laura Maccarana ; Department of Comparative Biomedicine and Food Science (BCA), University of Padova, Legnaro (PD), Italy
Giovanni Bittante ; Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Legnaro (PD), Italy
Jamie Newbold ; Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, SY23 3AL, United Kingdom

Abstract

The aim of this study was to investigate the rumen bacteria in terms of genetic biodiversity and variation due to different physical form of corn in cow diet. A total of twenty dry cows were fed for 3 months with the same diet, only differed for corn physical form, ten received corn grains, while the other ones received corn flour. To investigate the biodiversity of the bacterial 16S rRNA gene clone library analysis has been conducted and then the sequencing has been carried out using Ion Torrent PGM™ System. Bacterial population was tested using R statistical software. The Kruskal-Wallis one-way analysis of variance (Kruskal-Wallis, 1952) confirmed that the bacterial populations were different when the animals were fed grain compared with flour corn. Both the OUT’s abundance (Operational Taxonomic Unit) and the biodiversity indexes presented a significant difference among the two sample groups, underlining the large changes that take place even with small diet modifications in ruminal environment. There is still the need to deepen how exactly the diet changes the rumen phylogenetic structure and the consequences on bacteria’s activity.

Keywords

milk production; suckling kits; nursing

Hrčak ID:

150633

URI

https://hrcak.srce.hr/150633

Publication date:

2.9.2015.

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