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Agriculture, Vol. 21 No. 1 SUPPLEMENT, 2015.

Original scientific paper

https://doi.org/10.18047/poljo.21.1.sup.35

IMPACT OF SNPS IN CANDIDATE GENES ON ECONOMICALLY IMPORTANT TRAITS IN PINZGAU CATTLE

Anna Trakovická ; Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Tr. A. Hlinku 2, 94976 Nitra, Slovakia
Nina Moravčíková ; Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Tr. A. Hlinku 2, 94976 Nitra, Slovakia
Rudolf Nádaský ; Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Tr. A. Hlinku 2, 94976 Nitra, Slovakia
Radovan Kasarda ; Slovak University of Agriculture, Faculty of Agrobiology and Food Resources, Tr. A. Hlinku 2, 94976 Nitra, Slovakia

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Abstract

The aim of this work was to evaluate the impact of polymorphisms in bovine genes encoding leptin (LEP), leptin receptor (LEPR) and pituitary transcription factor (Pit-1) on economically important traits in Pinzgau cattle. The genomic DNA samples were taken from the total of 85 Pinzgau cows. The detection of LEP/Arg2059Cys, LEPR/ Thr945Met, and Pit-1/g.1178A>G single nucleotide polymorphisms were performed in order to evaluate their effect on long-life milk production traits. The genotyping of analysed animals was carried out using PCR-RFLP method. The observed allele frequencies showed that the LEPA, LEPRC and Pit-1B allele were the most distributed in the analysed population. The high proportion of homozygous genotype in case of each locus was transferred in to the relatively lower level of total observed heterozygosity which also confirmed the positive values of FIS index. The results of statistical analyses indicated the significant effect (P<0.05) of the evaluated polymorphisms in LEP and LEPR genes on milk, protein and fat yield. The LEPAA and LEPRCC genotypes seem to be a desirable for milk production improvement. For the Pit-1 gene only tendency to increase the milk production for AA homozygous animals was found out. Our study was biased mainly by sample size and therefore the analysis of greater population is recommended.

Keywords

LEP; LEPR; milk production; Pinzgau; Pit-1; SNP genotyping

Hrčak ID:

150667

URI

https://hrcak.srce.hr/150667

Publication date:

2.9.2015.

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