Short communication, Note
https://doi.org/10.17113/ftb.54.01.16.4041
16S rRNA in situ Hybridization Followed by Flow Cytometry for Rapid Identification of Acetic Acid Bacteria Involved in Submerged Industrial Vinegar Production
Janja Trček
; Faculty of Natural Sciences and Mathematics, University of Maribor, Maribor, Slovenia
Luka Lipoglavšek
; Biotechnical Faculty, University of Ljubljana, Domžale, Slovenia
Gorazd Avguštin
; Biotechnical Faculty, University of Ljubljana, Domžale, Slovenia
Abstract
Acetic acid bacteria are involved in many biotechnological processes such as vitamin C, gluconic acid, miglitol or acetic acid production, and others. For a technologist trying to control the industrial process, the ability to follow the microbiological development of the process is thus of importance. During the past few years hybridization in a combination with flow cytometry has often been used for this purpose. Since vinegar is a liquid, it is an ideal matrix for flow cytometry analysis. In this work we have constructed a specific probe for highly acetic acid-resistant species of the acetic acid bacteria and a protocol for in situ hybridization, which in combination with flow cytometry enables direct monitoring of bacteria producing vinegar with >10 % of acetic acid. The approach was successfully applied for monitoring microbiota during industrial vinegar production.
Keywords
microbiota; vinegar; acetic acid bacteria; 16S rRNA probe; in situ hybridization; flow cytometry
Hrčak ID:
155110
URI
Publication date:
31.3.2016.
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