Acta Botanica Croatica, Vol. 75 No. 1, 2016.
Original scientific paper
https://doi.org/10.1515/botcro-2016-0005
Species delimitation and genetic diversity analysis in Salvia with the use of ISSR molecular markers
Masoumeh Safaei
; Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
Masoud Sheidai
; Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
Behnaz Alijanpoor
; Iran National Botanic Garden, Tehran, Iran
Zahra Noormohammadi
; Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract
Thirty-nine plant specimens of six Salvia species were collected from different localities of the Alborz mountain region in Iran and studied for morphological and genetic variability and species relationship. Inter simple sequence repeats (ISSR) molecular markers showed a high degree of within-species and interspecific genetic variability in Salvia. Analysis of molecular variance and Hickory tests showed significant molecular difference among the studied populations. A principal coordinate analysis plot of morphological characters grouped the species into two distinct groups, supporting their taxonomic treatment. This was partly supported by ISSR networking. The Mantel test did not show a correlation between genetic distance and the geographical distance of the studied populations. STRUCTURE and reticulation analyses revealed some degree of gene flow among the species. The present study showed that ISSR molecular markers could be used in Salvia species delimitation along with morphological study.
Keywords
ISSR; network; population structure; Salvia
Hrčak ID:
155273
URI
Publication date:
1.4.2016.
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