Acta Botanica Croatica, Vol. 39 No. 1, 1980.
Original scientific paper
Further Investigation of the Defective Kazakhstan Strain of Tobacco Mosaic Virus
Davor Miličić
; Hrvatska
Mercedes Wrischer
; Hrvatska
Abstract
The paper presents some new data on host plants of the defective Kazakhstan strain (Ka) of tobacco mosaic virus. Chenopodium amaranticolor and some Nicotiana species can be used as local lesion hosts for detailed investigations. The appearance of various forms of intracellular inclusions during the infection process was analysed by means of light microscope.
The electron microscopic preparations of fresh infective sap treated with potassium phosphotungstate showed that the virus particles rarely had the length of 300 nm, more frequently they were shorter or longer. When before this treatment the infected leave’s are kept some hours in a refrigerator, the particles are mostly desintegrated. In the infection phase, when long coiled fibres are present in the cells the virus particles are often broken and consist of fragments about 100 nm long.
Moreover the ultrastructure of intracellular inclusions is described and illustrated. In the late phases of infection it was not possible to fix well various forms of cytoplasmic and intranuclear inclusions.
Although the Ka strain is defective, it can retain its infectivity in dried leaves for many years and maybe in this manner maintain itself in nature.
Keywords
Hrčak ID:
158358
URI
Publication date:
31.12.1980.
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