Original scientific paper

The Influence of Low Temperature on the Stability of Kazakhstan and Common Strain of Tobacco Mosaic Virus

Davor Miličić ; Hrvatska
Mercedes Wrischer ; Hrvatska

Abstract

The Kazakhstan strain (Ka strain) of tobacco mosaic virus is defective and particles of normal length of 300 nm are rarely found in preparations. However, particles over 1,000 nm long and many fragments shorter than 300 nm are very frequent. Such particles appeared in preparations made by negative staining method from living leaves kept at 21° C (Fig. 1).
As the Ka strain is sensitive to low temperatures, the form of the particles in preparations made from leaves kept for 14 hours at low temperature differs very much from those kept at normal temperature. The preparations from the material kept at 10° C mostly contained particles at various stages of decomposition (Fig. 2).
For comparison leaves infected with common TMV were treated in the same manner. Many normal particles of 300 nm were found in the preparations from leaves kept at 2T C (Fig. 3). However, in the preparations from the material kept at 10° C, many particles were decomposed. Nevertheless, the largest number of particles had preserved the normal form and length of 300 nm (Fig. 4).

Keywords

Hrčak ID:

158405

URI

https://hrcak.srce.hr/158405

Publication date:

31.12.1981.

Article data in other languages: croatian

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