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Original scientific paper

The Effect of 2,4-D and Kinetin on Soybean Calus Culture

Zdenko Rengel ; Hrvatska
Sibila Jelaska ; Hrvatska


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Abstract

Long-term callus cultures of barley (Hordeum vulgare L.) with decreased embryogenic potential were used in testing the influence of L-proline on somatic embryogenesis. The addition of up to 10 mM L-proline to the induction medium increased both the frequency of embryoid formation and the number of subsequently regenerated plantlets. Most of the plantlets (82%), however, were albinos. Some callus lines retained embryoid and plantlet formation capacity after being in culture for more than 21 months.

Keywords

Hrčak ID:

159210

URI

https://hrcak.srce.hr/159210

Publication date:

31.12.1986.

Article data in other languages: croatian

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