Original scientific paper

Peroxidases in Pumpkin Embryogénie Callus Lines

Marijana Krsnik Rasol ; Hrvatska
Sibila Jelaska ; Hrvatska
Draško Šerman ; Hrvatska

Abstract

Peroxidase isozymes and their total activity have ben studied in pumpkin embryogenic callus lines DE and NA3 in order to state how peroxidases are influenced by different kinds of exogenous growth regulators.
Peroxidase bands Rm 0.46 and 0.54—0.56 were present in both lines regardless of the growth regulator. NAA and adenine sulphate induced the enzyme activity at the position Rm 0.35 which disappeared if the tissue was growing on 2,4-D.
The changes in isoperoxidases pattern preceded any morphological change in the culture. The total peroxidase activity increased with time in line NAs, however, it remained low in line DE. Protein content changed inversely with peroxidase activity.

Keywords

Hrčak ID:

159215

URI

https://hrcak.srce.hr/159215

Publication date:

31.12.1986.

Article data in other languages: croatian

Visits: 1.191 *