Original scientific paper

In vitro Clonal Multiplication of Cucurbita pepo by Single-node Culture

Biserka Juretić ; Hrvatska
Vesna Katavić ; Hrvatska
Sibila Jelaska ; Hrvatska

Abstract

Pumpkin (Cucurbita pepo L.) plantlets originated from (6-8 -day-old) seedling shoot tips and somatic embryos — most of them from long-term callus lines, were cloned by nodal explants on agar solidified MS medium without hormones or MS containing IAA and/or BA. The effects of exogenous growt regulators on shoot elongation, root formation and rate of multiplication were investigated. The best development of shoots derived from seedling explants was achieved on MS supplemented with 2.9 pM IAA and 0.22 pM BA, and from somatic embryo shoot explants on MS medium with 5.7 pM IAA. Root formation was obtained on MS without hormones, and MS supplemented with 5.7 pM IAA, or with 2.9 pM IAA and 0.22 pM BA. The last combination of hormones induced callus proliferation at the basal end of shoots but more abundant roots than on medium without BA.

Keywords

Hrčak ID:

159504

URI

https://hrcak.srce.hr/159504

Publication date:

31.12.1989.

Article data in other languages: croatian

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