Croatica Chemica Acta, Vol. 89 No. 1, 2016.
Original scientific paper
https://doi.org/10.5562/cca2902
Spectrophotometric Determination of Malondialdehyde in Urine Suitable for Epidemiological Studies
Tin Weitner
; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Suzana Inić
; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Jasna Jablan
orcid.org/0000-0003-4741-0819
; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Mario Gabričević
; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Ana-Marija Domijan
orcid.org/0000-0001-5645-9732
; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Abstract
A reliable method for spectrophotometric determination of urinary malondialdehyde (MDA), according to the thiobarbituric acid (TBA) assay, is described. To account for matrix interference and differences in individual urine composition, standard addition procedure was applied. The method is adequately selective (LoQ = 0.09 μM in the presence of 0.1 M creatinine and 0.5 M urea) and reliable (within-day and between-day variability of less than 5 %). The mean level of urinary MDA was 1.52 ± 0.73 µM that is in good agreement with spectrofluorometric determination (1.20 ± 0.56 μM; p = 0.085) as well as with previous studies that used HPLC. Furthermore, it is demonstrated that MDA is stabile in urine at room temperature for 24 h and when stored at –20 °C for 6 months. The described method enables simple, rapid and cost-effective determination of urinary MDA as a relevant and non-invasive marker of “whole-body” oxidative stress.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Keywords
malondialdehyde; thiobarbituric acid assay; urine; interferences; stability
Hrčak ID:
160091
URI
Publication date:
20.6.2016.
Visits: 5.367 *