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https://doi.org/10.5562/cca2902

Spectrophotometric Determination of Malondialdehyde in Urine Suitable for Epidemiological Studies

Tin Weitner ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Suzana Inić ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Jasna Jablan orcid.org/0000-0003-4741-0819 ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Mario Gabričević ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Ana-Marija Domijan orcid.org/0000-0001-5645-9732 ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia

Sažetak

A reliable method for spectrophotometric determination of urinary malondialdehyde (MDA), according to the thiobarbituric acid (TBA) assay, is described. To account for matrix interference and differences in individual urine composition, standard addition procedure was applied. The method is adequately selective (LoQ = 0.09 μM in the presence of 0.1 M creatinine and 0.5 M urea) and reliable (within-day and between-day variability of less than 5 %). The mean level of urinary MDA was 1.52 ± 0.73 µM that is in good agreement with spectrofluorometric determination (1.20 ± 0.56 μM; p = 0.085) as well as with previous studies that used HPLC. Furthermore, it is demonstrated that MDA is stabile in urine at room temperature for 24 h and when stored at –20 °C for 6 months. The described method enables simple, rapid and cost-effective determination of urinary MDA as a relevant and non-invasive marker of “whole-body” oxidative stress.

This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi

malondialdehyde; thiobarbituric acid assay; urine; interferences; stability

Hrčak ID:

160091

URI

https://hrcak.srce.hr/160091

Datum izdavanja:

20.6.2016.

Posjeta: 6.328 *