Original scientific paper

Efficient shoot organogenesis of begonia (Begonia rex Putz.) induced by thidiazuron

Sibila Jelaska ; University of Zagreb, Faculty of Science, Department of Molecular Biology, Zagreb, Croatia
Nataša Bauer ; University of Zagreb, Faculty of Science, Department of Molecular Biology, Zagreb, Croatia
Ela Kosor ; University of Zagreb, Faculty of Science, Department of Molecular Biology, Zagreb, Croatia
Dunja Leljak ; University of Zagreb, Faculty of Science, Department of Molecular Biology, Zagreb, Croatia

Abstract

The effects of thidiazuron (TDZ) on adventitious shoot bud formation from leaf and petiole explants excised from in-vitro-grown plantlets of a begonia were tested by a two-step procedure: a short exposure (5 to 120 min) to 40 pM TDZ in liquid MS medium followed by culture on agar-solidified MS medium without growth regulators. Exposure of explains for five min to TDZ induced bud formation, but the number of buds was increased with prolonged exposure. Sixty min exposure to TDZ resulted in the highest mean number of regenerated shoots (24/leaf disc: 44/petiole segment after five weeks of culture). Shoots generated after two weeks in culture without, or with a small amount of, callus. During the third and fourth week in culture shoots developed small roots, and from the mother explant separated shoots elongated and further developed. The sucrose concentration in the basal expression medium influenced the efficiency of shoot organogenesis. The frequency of shoot formation tended to increase when the medium was supplemented with 2% sucrose instead with 3%.

Keywords

Begonia rex; leaf disc; micropropagation; organogenesis; petiole segment; thidiazuron

Hrčak ID:

160833

URI

https://hrcak.srce.hr/160833

Publication date:

31.12.2001.

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