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Food Technology and Biotechnology, Vol. 54 No. 3, 2016.

Original scientific paper

https://doi.org/10.17113/ftb.54.03.16.4211

Effect of Tween 80 and Acetone on the Secretion, Structure and Antioxidant Activities of Exopolysaccharides from Lentinus tigrinus

Peixin He ; School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China
Shuangshuang Wu ; School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China
Lige Pan ; School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China
Siwen Sun ; School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China
Duobin Mao ; School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China
Chunping Xu ; School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China

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Abstract

In this study, the effects of the addition of Tween 80 and acetone on secretion, structure and antioxidant activities of Lentinus tigrinus exopolysaccharides (EPS) were investigated. It was found that Tween 80 and acetone displayed a stimulatory effect on EPS secretion. The EPS obtained by the addition of Tween 80 (EPS-T), acetone (EPS-A) and control (EPS-C) were purified by Sepharose CL-6B gel filtration chromatography and molecular mass of purified fractions was estimated to be 22.1, 137 and 12 kDa, respectively. Monosaccharide composition analysis indicated that EPS-T, EPS-A and EPS-C were mainly composed of glucose and mannose. Congo Red test indicated that EPS-T and EPS-A had a highly ordered conformation of triple helix, while EPS-C had a random coil conformation. Furthermore, EPS-A exhibited higher DPPH scavenging and antiproliferative activities than EPS-C and EPS-T, which might be attributed to the molecular mass.

Keywords

exopolysaccharides; Lentinus tigrinus; molecular mass; molecular conformation; antiproliferative activity

Hrčak ID:

165240

URI

https://hrcak.srce.hr/165240

Publication date:

8.9.2016.

Article data in other languages: croatian

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