Short communication, Note

https://doi.org/10.1515/botcro-2016-0050

Microsatellite allele length variations in inter-specific hybrids of Eucalyptus

Murugan Sumathi ; Institute of Forest Genetics and Tree Breeding Coimbatore 641 002 Tamilnadu, India
Yasodha Ramasamy ; Institute of Forest Genetics and Tree Breeding Coimbatore 641 002 Tamilnadu, India

Abstract

The genus Eucalyptus encompasses several species of industrial importance. Many of these species have been subjected to genetic characterization using different kinds of DNA markers. More than 1000
microsatellites have been identified from the genome of eucalypts and they are highly amenable for cross species transferability. During cross amplifi cation of microsatellites, homoplasy is reported in many species in which although the allele size might be the same, the sequences are not. Thus, it is essential to ascertain the DNA sequence homology with source and target microsatellite repeats. Accordingly, fifty five alleles from six microsatellite loci (ECc1, ECc2, Eg61, Embra100, Embra1468 and Embra2002) were amplified in two inter-specifi c hybrid populations (Eucalyptus tereticornis × E. grandis and E. tereticornis × E. camaldulensis) and sequenced. The results showed that all the microsatellite loci were amplifying the target repeat types except
for the loci Eg61 and Embra2002. The locus Eg61 has target repeat of (CAA)(GAT) but the sampled alleles had either (CAA)(GAT) or (GAT) alone. Similarly, the Embra2002 locus was targeting interrupting repeats
of (CCA)..(CCA), but the sequenced alleles had repeats of (CCA) with or without interruption. Nevertheless, the allele size estimated in electrophoresis for hybrids was in conformity with that of the parent
alleles. This study suggests the need for validation of the repeat characteristics of microsatellites by sequencing of the alleles particularly in cross species amplification.

Keywords

allele sequences; Eucalyptus: inter-specific hybrids; microsatellites; repeat size

Hrčak ID:

179965

URI

https://hrcak.srce.hr/179965

Publication date:

1.4.2017.

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