Sjemenarstvo, Vol. 24 No. 2, 2007.
Original scientific paper
DEVELOPMENT OF RAPID MICROPROPAGATION METHOD OF Aloe vera L.
S. Ahmed
A.H. KABIR
M.B. Ahmed
M.A. RAZVY
S. GANESAN
Abstract
An efficient micropropagation method has been developed using shoot tip explants in Aloe vera L. The process involves subsequent in vitro morphogenesis and rooting of the in vitro proliferated shoots and transplantation of regenerated plants under ex vitro condition. Shoot proliferation was found best in MS medium containing BA 2.0 mg/l, KIN 0.5 mg/l and NAA 0.2 mg/l. Maximum 98.96% shoots were proliferated in this media composition. This media composition is best comparing to other treatment used in this study. Highest shoot number per explant was also achieved in the same medium within 5 weeks. In case of adventitious rooting, MS medium containing NAA 0.2 mg/l and NAA 0.5 mg/l was found to be the best. Maximum 80.25% rooting and highest number of root per culture (6.71) was obtained in this media composition. After transplanting the 20 days old rooted shoots into mixture of garden soil, compost and sand (2:1:1), 80% of survivability after 5 weeks was an achieved. Regenerated plants after acclimatization were transferred to soil and they showed 82% survival. The regenerated plants were morphologically similar to the mother plants.
Keywords
Shoot tips; MS medium; shoot proliferation; acclimatization; Aloe vera L
Hrčak ID:
18326
URI
Publication date:
28.11.2007.
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