Original scientific paper

https://doi.org/10.2478/botcro-2018-0004

Modulation of arsenic-induced oxidative stress and protein metabolism by diphenyleneiodonium, 24-epibrassinolide and proline in Glycine max L.

Vibhuti Chandrakar ; School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur 492 010, India
Amit Dubey ; Central Laboratory Facility, Chhattisgarh Council of Science and Technology, Raipur 492 010, India
Sahu Keshavkant orcid.org/0000-0003-3679-479X ; School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur 492 010, India

Abstract

Arsenic (As)-toxicity is a major constraint for crop production. The present study was intended to examine the comparative ameliorative effects of diphenyleneiodonium (DPI), 24-epibrassinolide (EBL) and proline (Pro) on As-stress in Glycine max L. Seeds of Glycine max L. were subjected to As (100 μM) singly, and together with DPI (10 μM), EBL (0.5 μM) or Pro (10 mM), for five days, and were then analyzed. Experimental results showed that As treatment caused a substantial fall in growth traits like germination percentage, radicle length and dry mass, which was accompanied by As accumulation. Additionally, As application also revealed reduced viability, total protein content and activities of antioxidative enzymes (superoxide dismutase, catalase and ascorbate peroxidase), while it increased the levels of total sugar, proline and oxidative stress markers such as electrolyte leakage, reactive oxygen species, lipid oxidized products, protein carbonyls and hydroperoxides, Amadori and Maillard reaction products, malondialdehyde-/4-hydroxy-2-nonenal-protein adducts, protease and proteasome. Isozymes of antioxidative enzymes were also observed to be altered considerably under As-stress. Impressively, DPI, EBL and Pro played their role as protective agents, hence caused enhanced growth and reduced As accumulation. These protective chemicals also improved the viability, accruals of total protein, total sugar and endogenous proline, and activities of antioxidants, while they reduced the levels of oxidative stress markers. Our findings demonstrated the involvement of DPI, EBL and Pro in As-stress tolerance in Glycine max L. Further, Pro appears to be superior to DPI and EBL, in alleviating As-induced responses in Glycine max L.

Keywords

arsenic; diphenylene iodonium; 24-epibrassinolide; oxidative stress; proline; protein metabolism; reactive oxygen species

Hrčak ID:

198417

URI

https://hrcak.srce.hr/198417

Publication date:

1.4.2018.

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