Original scientific paper

IMPROVED SOMATIC EMBRYOGENESIS USING L-ASPARAGINE IN WHEAT (Triticum aestivum L.)

K. K. Sarker ; Biotechnology and Microbiology Laboratory, Department of Botany, Rajshahi University, Bangladesh
A. H. Kabir
S. A. Sharmin
Z. Nasir
M. F. Alam

Abstract

Somatic embryos and plants were produced from mature and immature embryo derived callus of wheat. Immature embryo showed a better capacity for somatic embryogenesis and plant regeneration. Presence of 2, 4-D was shown to be essential for induction and maintenance of somatic embryogenesis. Organic nitrogenous additives (L-proline, L-asparagine and casein hydrolysate) marked an effect on somatic embryo formation when they were used in the callus maintenance medium. Histological analysis confirmed the formation of somatic embryo. The regenerated plants had the same morphology as the original plants.

Keywords

Immature embryo; somatic embryo; histology and organic nitrogen; additives

Hrčak ID:

22427

URI

https://hrcak.srce.hr/22427

Publication date:

12.3.2008.

Article data in other languages: croatian

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