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Original scientific paper

ABCB1, CYP2B6, and CYP3A4 genetic polymorphisms do not affect methadone maintenance treatment in HCV-positive patients

Davorka Sutlović orcid id ; University of Split Department for Health Studies, Split, Croatia 2 University of Split School of Medicine, Department of Toxicology and Pharmacogenetics, Split, Croatia
Željko Ključević ; Public Health Institute of the Split-Dalmatia County, Department of Mental Health, Prevention and Outpatient Addiction Treatment, Split, Croatia
Sendi Kuret ; University of Split Department for Health Studies, Split, Croatia, 4 University Hospital Centre Split, Department of Pathology and Forensic Medicine, Split, Croatia

Full text: english pdf 375 Kb

page 353-358

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Full text: croatian pdf 375 Kb

page 358-358

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The aim of this study was to determine the influence of ABCB1, CYP2B6, and CYP3A4 genetic polymorphisms on methadone metabolism in patients with hepatitis C virus (HCV) undergoing methadone maintenance treatment (MMT).The study included 35 participants undergoing MMT, who were divided in three groups: HCV-positive (N=12), HCVnegative (N=16), and HCV clinical remission (CR) (N=7). The concentrations of methadone and its main metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) were determined with gas chromatography-mass spectrometry. The patients were genotyped for ABCB1 rs1045642, CYP2B6 rs3745274, CYP3A4 rs2242480, and CYP3A4 rs2740574 polymorphisms. Differences between single nucleotide polymorphism (SNP) genotypes and methadone-to-EDDP ratio were analysed with one-way ANOVA, which showed no significant difference between the genes (p=0.3772 for ABCB1 rs1045642, p=0.6909 for CYP2B6 rs3745274, and p=0.6533 for CYP3A4 rs2242480). None of the four analysed SNP genotypes correlated with methadone-to-EDDP concentration ratio. A major influence on it in hepatitis C-positive patients turned out to be the stage of liver damage.


EDDP, genotyping, hepatitis C, liver damage, SNP

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Article data in other languages: croatian

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