Original scientific paper
Chitinase and Antifungal Activity of Endophytic Fungi Isolated from Hedychium coronarium J. Koenig
Anna Mendrofa
; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, North Sumatra
Erman Munir
; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, North Sumatra
Yurnaliza Yurnaliza
; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, North Sumatra
Anisa Lutfia
; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, North Sumatra
Adrian Hartanto
orcid.org/0000-0002-7971-2160
; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, North Sumatra
Abstract
Chitinase (EC 3.2.1.14) is a class of hydrolytic enzyme capable of degrading polymeric chitin, and it has been reported and isolated from many sources. In the present study, we evaluated the ability of endophytic fungi isolated from native Hedychium coronarium J. Koenig in producing chitinase following its characterization and the test of its inhibitory activities against Fusarium oxysporum Schlecht. emend. Snyder & Hansen, a pathogenic fungus causing wilt disease among common corps in North Sumatra. Twelve endophytic fungal isolates were tested for their ability to grow on chitin agar medium, resulting in only two isolates, namely isolate JRE 1A and JRE 4B, capable of producing a visible clear zone indicating the secretion of extracellular chitinase. The two isolates were observed for their morphology and identified on the basis of molecular markers (ITS1–ITS4) within the region of ITS-rDNA, resulting in the species designation of Trichoderma afroharzianum JRE 1A and Aspergillus fumigatus JRE 4B. The production was detected highest after 4 days of incubation for A. fumigatus JRE 4B (4.76 U/mL) and 3 days for T. afroharzianum JRE 1A (4.15 U/mL). The crude chitinases showed stable activities within the pH range of 4–7, stimulated by Mn2+ and Zn2+ and slightly inhibited by K+ and Ca2+. Furthermore, the strongest antagonistic activity against F. oxysporum was displayed by T. afroharzianum (CGI > 70%) followed by A. fumigatus (CGI > 30%). The precipitated crude chitinases extracted from the endophytes also inhibited the growth of the tested phytopathogenic fungi.
Keywords
biocontrol, chitinase, Fusarium oxysporum, ITS-rDNA
Hrčak ID:
259581
URI
Publication date:
29.6.2021.
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