Original scientific paper
PREVENTION OF GENOTOXIC EFFECT OF OCHRATOXIN A AND AFLATOXIN B1 BY ECHINACEA PURPUREA EXTRACTS
Huska Jukić
orcid.org/0000-0001-8196-778X
; University of Bihać, Faculty of Health Studies, Nositelja hrvatskog trolista 4, 77000 Bihać, Bosnia and Herzegovina
Maja Šegvić Klarić
; University of Zagreb, Faculty of Pharmacy and Biochemistry, Schrottova 39/I, 10000 Zagreb, Croatia
Nevenka Kopjar
; Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000 Zagreb, Croatia
Antun Jozinović
orcid.org/0000-0001-9627-1013
; Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 18, 31000, Osijek, Croatia
Tomislav Bogdanović
; Public Institution “Nature Park Kopački Rit”, Mali Sakadaš 1, 31327 Kopačevo, Bilje, Croatia
Drago Šubarić
orcid.org/0000-0002-6956-5814
; Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 18, 31000, Osijek, Croatia
Ivan Kosalec
; University of Zagreb, Faculty of Pharmacy and Biochemistry, Schrottova 39/I, 10000 Zagreb, Croatia
Abstract
The aim of this work was to examine the polyphenol content in Echinacea purpurea (L.) Moench extracts and to determine their antioxidant and antigenotoxic effect. The antioxidant activity of extracts and main components is determined by the application of electronic spin resonance and spectophotometric methods of capturing DPPH, superoxide and hydroxyl radical. The antigenotoxic effect was evaluated with genotoxic mycotoxins – aflatoxin B1 (AFB1) and ochratoxin A (OTA). Herbe ehinacea extract contained 13.31% of total polyphenols. Ehinacea extract contained a sufficient amount of phenolic acids (primarily chicoric and caftaric acid). Ehinacea extract showed the ability to neutralize DPPH radicals (EC50 = 15.67 μg/mL). Similar results were also identified by testing the capture of hydroxyl and superoxide radicals. The strong antioxidant activity of the dominant components that indicate phenolic acids as the ingredients that contribute the most to the antioxidant and antigenotoxic effect of echinacea extract was also found. Genotoxic suppression of AFB1 and OTA was established by a comet test, establishing a significant reduction in tail, tail intensity and tail torque in leukocytes co-treated with mycotoxins and different concentrations of extracts.
Keywords
antioxidant activity; comet assay; Echinacea purpurea; genotoxicity; polyphenols
Hrčak ID:
269616
URI
Publication date:
29.12.2021.
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