Preliminary communication
A Method for Histological, Enzyme Histochemical and Immunohistochemical Analysis of Periapical Diseases on Undecalcified Bone with Teeth
Maja Kovačević
Tomislav Tamarut
Sanja Zoričić
Snježana Bešlić
Abstract
The objective was to examine and apply a method for serial sectioning of undecalcified canine teeth with surrounding jawbone and to analyse it histologically, enzyme histochemically and immunohistochemically. Pulpitis and apical periodontitis were induced in a mongrel dog’s premolar teeth by exposing the pulp to the oral environmental influence for 50 days. After animal sacrifice undecalcified bone with the decoronated experimental teeth were embedded in methylmethacrylate and sectioned with a tungsten carbide knife at 5-7 μm. Sections were stained with toluidin blue (TB) for histological analysis and by a method of staining non-specific acid phosphatase (ACP), and tartrat-resistant acid phosphatase (TRAP). Immunohistochemical staining was performed for detection of CD 45+ lymphocytes. Because the specimens were 5-7 μm thick it was possible to perform a detailed cytological analysis of changes in the pulp and periapex in situ with a light microscope on histological and enzyme histochemical specimens. Immunohistochemical staining was not satisfactory. The method of embedding undecalcified bone and teeth in methylmethacrylate and sectioning with a tungstencarbide knife is satisfactory for histological and some enzyme histochemical analysis in endodontic research. However, immunohistochemical staining needs improvement.
Keywords
histology; dogs; methyl-methacrylate; periapical diseases; undecalcified bone
Hrčak ID:
2717
URI
Publication date:
15.9.2003.
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