Acta Pharmaceutica, Vol. 72 No. 4, 2022.
Original scientific paper
https://doi.org/10.2478/acph-2022-0033
Effect of in vitro gastrointestinal digestion on the chemical composition and antioxidant properties of Ginkgo biloba leaves decoction and commercial capsules
YUBO ZHOU
orcid.org/0000-0001-6281-2379
; College of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, Zhejiang, 315100, China
YINGXIN YANG
; College of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, Zhejiang, 315100, China
MINYAN MA
; College of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, Zhejiang, 315100, China
LINGYUN XIE
AIJUAN YAN
; College of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, Zhejiang, 315100, China
WEN CAO
; College of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, Zhejiang, 315100, China
Abstract
In this study Ginkgo biloba leaves (GBL) decoction and commercial capsules were digested using an in vitro model. Thirty-six active compounds were identified and quantified by HPLC-ESI-MS analysis based on the MS/MS patterns (precursor ions and product ions) and retention times, in comparison with reference standards. Most compounds in GBL showed a significant decrease during intestinal digestion, with an exception of vanillic acid and biflavonoids. Bioaccessibility values of chemical compositions varied between decoction and capsules samples. Also, significant reductions of total flavonoids and total phenolic content was observed after in vitro digestion. Both, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavenging capacity decreased after gastric digestion, but increased during intestinal digestion. Nevertheless, different behaviour was observed in reducing antioxidant power (FRAP) assay. Compared to the pH of digestion, the influence of digestive enzymes on the chemical composition and antioxidant activity of GBL was relatively minor. Overall, these results may help provide a valid foundation for further investigations on bioactive compounds and the pharmacodynamics of GBL.
Keywords
Ginkgo biloba L.; in vitro digestion; HPLC-MS/MS; chemical composition; antioxidant property; bioaccessibility
Hrčak ID:
273436
URI
Publication date:
31.12.2022.
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