Conference paper

Quality Control During Aminoacyl-tRNA Synthesis

M. Prćtorius-Ibba
S. Ataide
C. Hausmann
J. Levengood
J. Ling
S. Wang
H. Roy
M. Ibba

Abstract

The fidelity of translation is determined at two major points: the accuracy of aminoacyl-tRNA selection by the ribosomes and synthesis of cognate amino acid/tRNA pairs by aminoacyl-tRNA synthetases (aaRSs) in the course of the aminoacylation reaction. The most important point in aminoacylation is the accurate recognition of cognate substrates coupled with discrimination of
non-cognates. While this is generally accomplished by a single enzyme, we have recently found that discrimination against lysine analogues requires the existence of two unrelated lysyl-tRNA synthetases. For other amino acids, initial recognition is not sufficiently accurate with errors being
corrected by an intrinsic editing activity. Recent studies indicate how editing prevents the misinterpretation of phenylalanine as tyrosine in the genetic code and have shown the importance of this process in vivo. More recent studies indicate that while these editing reactions are critical in
the cytoplasm, some are absent from mitochondria suggesting that the overall fidelity of protein synthesis might be reduced in this compartment.

Keywords

Aminoacyl-tRNA; editing, genetic code; transfer RNA; translation

Hrčak ID:

2740

URI

https://hrcak.srce.hr/2740

Publication date:

10.3.2006.

Article data in other languages: croatian

Visits: 1.296 *