Original scientific paper

DISTINCTION OF TWO SPECIES OF CECIDOMYIES (DIPTERA: CECIDOMYIIDAE) REVEALED BY MITOCHONDRIAL DNA GENES VARIATION

Maha Mezghani Khemakhem ; Laboratoire de Genetique Moleculaire, Immunologie et Biotechnologie, Faculte des Sciences de Tunis, Tunis, Tunisie
Hanem Makni ; Laboratoire de Genetique Moleculaire, Immunologie et Biotechnologie, Faculte des Sciences de Tunis, Tunis, Tunisie
Mohamed Marrakchi ; Laboratoire de Genetique Moleculaire, Immunologie et Biotechnologie, Faculte des Sciences de Tunis, Tunis, Tunisie

Abstract

Mayetiola is an important pest of cereal in Tunisia. Two species were identified using isoenzymatic markers. To develop a DNA diagnostic tool, we assessed mitochondrial DNA study based on Restriction Fragment Length Polymorphism in Polymerase Chain Reaction Amplified Fragments (PCR -RFLP). Specific primers were used to amplify the cytochrome b and a 3’ terminal region of the 12S mt rDNA genes. Results showed that Sau3A restriction patterns can distinguish between the two morphologically similar species. Thus PCR-RFLP is a useful tool for identifying morphologically simiIar species of Mayetiola in large-scale pest identification.

Keywords

Mitochondrial DNA; PCR-RFLP; Diptera; Cecidomyiidae; Mayetiola; Tunisie

Hrčak ID:

284078

URI

https://hrcak.srce.hr/284078

Publication date:

31.1.2002.

Article data in other languages: croatian

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